ZNF24 Rabbit PolymAb, Unconjugated

Artikelname:	ZNF24 Rabbit PolymAb, Unconjugated
Artikelnummer:	ABB-A25460PM
Hersteller Artikelnummer:	A25460PM
Alternativnummer:	ABB-A25460PM-20UL, ABB-A25460PM-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	KOX17, RSG-A, ZNF191, ZSCAN3, Zfp191, ZNF24

Enables DNA-binding transcription activator activity, RNA polymerase II-specific, identical protein binding activity, and sequence-specific DNA binding activity. Involved in negative regulation of transcription, DNA-templated and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm.

Molekulargewicht:	42kDa
NCBI:	7572
UniProt:	P17028
Reinheit:	Affinity purification
Target-Kategorie:	ZNF24

Application Verdünnung:	WB,1:500 - 1:1000\|IF/ICC,1:50 - 1:200\|IF-P,1:50 - 1:200\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors.

	Western blot analysis of Jurkat cells usingZNF24 Rabbit PolymAb (A25460PM) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid tissue usingZNF24 Rabbit PolymAb(A25460PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast tissue usingZNF24 Rabbit PolymAb(A25460PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingZNF24 Rabbit PolymAb(A25460PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat brain tissue usingZNF24 Rabbit PolymAb(A25460PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of K-562 cells usingZNF24 Rabbit PolymAb (A25460PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging ofparaffin-embedded Mouse colon tissue usingZNF24 Rabbit PolymAb (A25460PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging ofparaffin-embedded Rat kidney tissue usingZNF24 Rabbit PolymAb (A25460PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging of A-431 cells usingZNF24 Rabbit PolymAb (A25460PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.