DDX1 Rabbit mAb, Unconjugated

Artikelname:	DDX1 Rabbit mAb, Unconjugated
Artikelnummer:	ABB-A25806
Hersteller Artikelnummer:	A25806
Alternativnummer:	ABB-A25806-100UL, ABB-A25806-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IHC-P, IP, WB
Spezies Reaktivität:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	DBP-RB, UKVH5d, DDX1

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that acts as an ATP-dependent RNA helicase that has been found to promote coronaviruses replication.

Molekulargewicht:	82 kDa
NCBI:	1653
UniProt:	Q92499
Reinheit:	Affinity purification
Target-Kategorie:	DDX1

Application Verdünnung:	WB,1:10000 - 1:60000\|IHC-P,1:1000 - 1:5000\|IP,0.5µg-4µg antibody for 400µg-600µg extracts of whole cells\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding.

	Western blot analysis of lysates from HeLa cells using DDX1 Rabbit mAb (A25806) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.8s.
	Western blot analysis of lysates from Mouse brain using DDX1 Rabbit mAb (A25806) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of lysates from Rat brain using DDX1 Rabbit mAb (A25806) at 1:20000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1 s.
	Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using DDX1 Rabbit mAb (A25806) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using DDX1 Rabbit mAb (A25806) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using DDX1 Rabbit mAb (A25806) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DDX1 Rabbit mAb (A25806) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using DDX1 Rabbit mAb (A25806) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunoprecipitation of DDX1 from 400 µg extracts of Rat brain tissue was performed using 0.5 µg of DDX1 Rabbit mAb (A25806). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DDX1 Rabbit mAb (A25806) at a dilution of 1:5000.