IFN-beta Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A25818
Artikelname: IFN-beta Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A25818
Hersteller Artikelnummer: A25818
Alternativnummer: ABB-A25818-100UL,ABB-A25818-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Mouse
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: Ifb, IFNB, If1da1, IFN-beta
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation, cellular response to virus, and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound, defense response to other organism, and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
Klonalität: Monoclonal
Molekulargewicht: 22kDa
NCBI: 15977
UniProt: P01575
Reinheit: Affinity purification
Target-Kategorie: Ifnb1
Application Verdünnung: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Mouse. ResearchArea: Cardiovascular Angiogenesis Cytokines OtherImmunology Innate Immunity Cytokines InterferonsCancer Tumor immunology Cytokines InterferonsNeuroscience DiseasesNeuroscience Processes. Shipping: Ice Bag
Western blot analysis of lysates from RAW 264.7 cells using IFN-beta Rabbit mAb (A25818) at 1:1000 dilutionincubated overnight at 4°C. Raw264.7 cells were treated with DMXAA(50 ug/mL) for 7 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.