Cytokeratin 17 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Cytokeratin 17 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A26619
• Hersteller Artikelnummer: A26619
• Alternativnummer: ABB-A26619-1000UL,ABB-A26619-20UL,ABB-A26619-100UL,ABB-A26619-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PC, K17, PC2, 39.1, CK-17, PCHC1

Produktbeschreibung

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.

Produkteigenschaften

• Molekulargewicht: 48kDa
• NCBI: 3872
• UniProt: Q04695
• Reinheit: Affinity purification
• Sequenz: NRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
• Target-Kategorie: KRT17

Anwendungsbeschreibung

• Application Verdünnung: WB,1:25000 - 1:150000|IF/ICC,1:3200 - 1:12800|IF-P,1:3200 - 1:12800|IHC-P,1:200 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Extracellular Matrix,Keratin.

Bilder

Western blot analysis of lysates from Mouse thymus using Cytokeratin 17 Rabbit mAb (A26619) at 1:25000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Cytokeratin 17 Rabbit mAb (A26619) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Western blot analysis of various lysates using Cytokeratin 17 Rabbit mAb (A26619)at 1:25000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): A549
Exposure time: 5s.

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Cytokeratin 17 Rabbit mAb (A26619) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 17 Rabbit mAb (A26619) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse skin tissue using Cytokeratin 17 Rabbit mAb (A26619) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat skin tissue using Cytokeratin 17 Rabbit mAb (A26619) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Human lung cancer tissue using Cytokeratin 17 Rabbit mAb (A26619, dilution 1:3200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of HeLa cells using Cytokeratin 17 Rabbit mAb (A26619, dilution 1:3200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.