RYR2 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: RYR2 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A27242
• Hersteller Artikelnummer: A27242
• Alternativnummer: ABB-A27242-1000UL,ABB-A27242-500UL,ABB-A27242-100UL,ABB-A27242-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: RyR, ARVC2, ARVD2, RYR-2, VTSIP, VACRDS

Produktbeschreibung

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.

Produkteigenschaften

• Molekulargewicht: 565kDa
• NCBI: 6262
• UniProt: Q92736
• Reinheit: Affinity purification
• Target-Kategorie: RYR2

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:4000|IF/ICC,1:100 - 1:400|IF-P,1:100 - 1:400|IHC-P,1:100 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Neuroscience,Calcium Signaling,Cardiovascular,Hypoxia,Heart,Contractility,Cardiac arrhythmias.

Bilder

Western blot analysis of various lysates using RYR2 Rabbit mAb (A27242) at 1:1000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Rat skeletal muscle
Exposure time: 0.5s.

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human heart tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using RYR2 Rabbit mAb (A27242) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of 293T cells using RYR2 Rabbit mAb (A27242, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of paraffin-embedded Mouse heart tissue using RYR2 Rabbit mAb (A27242, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.