NLRP3 Rabbit mAb, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: NLRP3 Rabbit mAb, Unconjugated, Monoclonal
• Artikelnummer: ABB-A27260
• Hersteller Artikelnummer: A27260
• Alternativnummer: ABB-A27260-20UL,ABB-A27260-100UL,ABB-A27260-1000UL,ABB-A27260-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, CLR1.1, DFNA34, PYPAF1, AGTAVPRL

Produktbeschreibung

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5 UTR structures are suggested by available data, however, insufficient evidence is available to determine if all of the represented 5 UTR splice patterns are biologically valid.

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 118kDa
• NCBI: 114548
• UniProt: Q96P20
• Reinheit: Affinity purification
• Target-Kategorie: NLRP3

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Cancer,Cell Biology Developmental Biology,Apoptosis,Growth factors,Immunology Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,Cardiovascular. Shipping: Ice Bag

Bilder

Western blot analysis of lysates from U-937 cells using NLRP3 Rabbit mAb (A27260) at 1:1000 dilutionincubated overnight at 4°C. U-937 cells were treated by PMA/TPA (80 nM) at 37°C for overnight and LPS (1 µg/ml) at 37°C for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.