This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants.
Flow cytometry: 1X10 6 C57BL/6 mouse splenocytes (untreated,left) and C57BL/6 mouse splenocytes (treated with PMA 50ng/ml, Ionomycin 1ug/ml, Brefeldin A 5ug/ml for 6 h,right) were surface-stained with ABflo 488 Rat anti-Mouse CD3 mAb (A27161,5 µl/Test), and then intracellularly-stained with PE Rabbit anti-Mouse TNF-alpha mAb (A27472,5 µl/Test).
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