[KD Validated] NAT10 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: [KD Validated] NAT10 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A28373
• Hersteller Artikelnummer: A28373
• Alternativnummer: ABB-A28373-100UL,ABB-A28373-20UL,ABB-A28373-500UL,ABB-A28373-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ALP, Kre33, NET43

Produktbeschreibung

The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization.

Produkteigenschaften

• Molekulargewicht: 116 kDa/107 kDa
• NCBI: 55226
• UniProt: Q9H0A0
• Reinheit: Affinity purification
• Target-Kategorie: NAT10

Anwendungsbeschreibung

• Application Verdünnung: WB,1:3000 - 1:10000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|IF/ICC,1:200 - 1:800|IHC-P,1:4000 - 1:16000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,RNA Binding,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Cytoskeleton,Microtubules,Immunology Inflammation,Cytokines.

Bilder

Western blot analysis of lysates from wild type (WT) and NAT10 knockdown (KD) HeLa cells using [KD Validated] NAT10 Rabbit mAb (A28373) at 1:5000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10 s.

Western blot analysis of lysates from NIH/3T3 cells using [KD Validated] NAT10 Rabbit mAb (A28373) at 1:5000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10 s.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] NAT10 Rabbit mAb (A28373) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] NAT10 Rabbit mAb (A28373) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat skin tissue using [KD Validated] NAT10 Rabbit mAb (A28373) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of HeLa cells using [KD Validated] NAT10 Rabbit mAb (A28373, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of A549 cells using [KD Validated] NAT10 Rabbit mAb (A28373, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using [KD Validated] NAT10 Rabbit mAb (A28373, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunoprecipitation of NAT10 from 300 µg extracts of HeLa cells was performed using 2 µg of [KD Validated] NAT10 Rabbit mAb (A28373). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] NAT10 Rabbit mAb (A28373) at a dilution of 1:5000.