AHR Rabbit pAb, Unconjugated, Polyclonal

Produktübersicht

• Artikelname: AHR Rabbit pAb, Unconjugated, Polyclonal
• Artikelnummer: ABB-A28506
• Hersteller Artikelnummer: A28506
• Alternativnummer: ABB-A28506-1000UL,ABB-A28506-100UL,ABB-A28506-20UL,ABB-A28506-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Mouse
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: Ah, In, Ahh, Ahre, bHLHe76

Produktbeschreibung

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm, upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. Two transcript variants encoding different isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 95 kDa
• NCBI: 11622
• UniProt: P30561
• Reinheit: Affinity purification
• Target-Kategorie: Ahr

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:3000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure
• Anwendungsbeschreibung: Cross-Reactivity: Mouse. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cell Biology Developmental Biology,Apoptosis. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates using AHR Rabbit pAb (A28506) at 1:3000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Neuro-2a.
Exposure time: 45 s.