Carbonic Anhydrase 1 (CA1) Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A4406
Artikelname: Carbonic Anhydrase 1 (CA1) Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A4406
Hersteller Artikelnummer: A4406
Alternativnummer: ABB-A4406-20UL,ABB-A4406-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: CAB, CA-I, Car1, HEL-S-11, Carbonic Anhydrase 1 (CA1)
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Klonalität: Monoclonal
Molekulargewicht: 29kDa
NCBI: 759
UniProt: P00915
Reinheit: Affinity purification
Target-Kategorie: CA1
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Cardiovascular,Blood. Shipping: Ice Bag
Western blot analysis of various lysates using Carbonic Anhydrase 1 (CA1) (CA1) Rabbit mAb (A4406) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.