ERCC1 Rabbit mAb, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: ERCC1 Rabbit mAb, Unconjugated, Monoclonal
• Artikelnummer: ABB-A4971
• Hersteller Artikelnummer: A4971
• Alternativnummer: ABB-A4971-20UL,ABB-A4971-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: UV20, COFS4, RAD10, ERCC1

Produktbeschreibung

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5 incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 33kDa
• NCBI: 2067
• UniProt: P07992
• Reinheit: Affinity purification
• Target-Kategorie: ERCC1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Cancer,Tumor biomarkers. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates using ERCC1 Rabbit mAb (A4971) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.