Rad52 Rabbit mAb, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: Rad52 Rabbit mAb, Unconjugated, Monoclonal
• Artikelnummer: ABB-A5186
• Hersteller Artikelnummer: A5186
• Alternativnummer: ABB-A5186-100UL,ABB-A5186-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: RAD52, DNA repair protein RAD52 homolog, Rad52

Produktbeschreibung

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 13kDa/15kDa/25kDa/46kDa
• NCBI: 5893
• UniProt: P43351
• Reinheit: Affinity purification
• Target-Kategorie: RAD52

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Cell Biology Developmental Biology,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates using Rad52 Rabbit mAb (A5186) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Plus Kit (RM00021) .
Exposure time: 3min.