HEC1/NDC80 Rabbit pAb, Unconjugated, Polyclonal

Produktübersicht

• Artikelname: HEC1/NDC80 Rabbit pAb, Unconjugated, Polyclonal
• Artikelnummer: ABB-A5411
• Hersteller Artikelnummer: A5411
• Alternativnummer: ABB-A5411-20UL,ABB-A5411-100UL,ABB-A5411-500UL,ABB-A5411-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: HEC, HEC1, TID3, KNTC2, HsHec1, hsNDC80, HEC1/NDC80

Produktbeschreibung

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 74kDa
• NCBI: 10403
• UniProt: O14777
• Reinheit: Affinity purification
• Sequenz: ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVE
• Target-Kategorie: NDC80
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,Cancer,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates using HEC1/NDC80 Rabbit pAb (A5411) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.