[KO Validated] RAB1B Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A7514
Artikelname: [KO Validated] RAB1B Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A7514
Hersteller Artikelnummer: A7514
Alternativnummer: ABB-A7514-100UL,ABB-A7514-20UL,ABB-A7514-1000UL,ABB-A7514-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: RAB1B
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196], Alvarez et al., 2003 [PubMed 12802079]).
Klonalität: Polyclonal
Molekulargewicht: 22kDa
NCBI: 81876
UniProt: Q9H0U4
Reinheit: Affinity purification
Sequenz: YLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC
Target-Kategorie: RAB1B
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction,G protein signaling,Small G proteins. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and RAB1B knockout (KO)HeLa cellsusing[KO Validated] RAB1B Rabbit pAb RAB1B Rabbit pAb (A7514) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:1s.