Endo G Rabbit mAb, Unconjugated

Artikelnummer: ABB-A8801
Artikelname: Endo G Rabbit mAb, Unconjugated
Artikelnummer: ABB-A8801
Hersteller Artikelnummer: A8801
Alternativnummer: ABB-A8801-100UL, ABB-A8801-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: ENDOG, endonuclease G, Endo G
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Molekulargewicht: 33kDa
NCBI: 2021
UniProt: Q14249
Reinheit: Affinity purification
Target-Kategorie: ENDOG
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Invasion and Metastasis,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Cell Cycle,Endocrine Metabolism,Mitochondrial metabolism.
Western blot analysis of various lysates using Endo G Rabbit mAb (A8801) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.