Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag Affinity Gel}

Produktübersicht

• Artikelname: Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag Affinity Gel}
• Artikelnummer: ABB-AE120
• Hersteller Artikelnummer: AE120
• Alternativnummer: ABB-AE120-500UL, ABB-AE120-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Synonym: Myc, Myc tag, Myc-tag, Myc-Tag

Produktbeschreibung

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Produkteigenschaften

• Konzentration: IP
• Reinheit: Affinity purification
• Target-Kategorie: Myc

Anwendungsbeschreibung

• Application Verdünnung: IP,20µl-40µl Affinity Gel for 100µg-300µg extracts of whole cells
• Anwendungsbeschreibung: Cross-Reactivity: Species independent

Bilder

Immunoprecipitation of PTEN-Myc from 150 µg extracts of 293T cells transfected with a PTEN expression vector containing a single C-terminal Myc-Tag was performed using 20 µl of Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag Affinity Gel} (AE120). Affinity Gel-conjugated Rabbit IgG isotype control mAb was used to precipitate the Control IgG sample. The IP sample was eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using HRP-conjugated Rabbit anti Myc-Tag mAb (AE102) at a dilution of 1:5000.