Phospho-ErbB4/HER4-Y1284 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-ErbB4/HER4-Y1284 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0034
• Hersteller Artikelnummer: AP0034
• Alternativnummer: ABB-AP0034-500UL,ABB-AP0034-20UL,ABB-AP0034-1000UL,ABB-AP0034-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: HER4, ALS19, p180erbB4, Phospho-ErbB4/HER4-Y1284

Produktbeschreibung

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 147kDa
• NCBI: 2066
• UniProt: Q15303
• Reinheit: Affinity purification
• Sequenz: PEYLS
• Target-Kategorie: ERBB4
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation.

Bilder

Western blot analysis of lysates from MCF7 cells usingPhospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034) at1:700 dilution. MCF7 cells were treated with IGF (200 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:30s.