Phospho-ESRalpha-S118 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-ESRalpha-S118 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0146
• Hersteller Artikelnummer: AP0146
• Alternativnummer: ABB-AP0146-100UL, ABB-AP0146-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ER, ESR, Era, ESRA, ESTRR, NR3A1, Phospho-ESRalpha-S118

Produktbeschreibung

This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.

Produkteigenschaften

• Molekulargewicht: 66kDa
• NCBI: 2099
• UniProt: P03372
• Reinheit: Affinity purification
• Target-Kategorie: ESR1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|IF/ICC,1:100 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Nuclear Receptor Signaling,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway.

Bilder

Western blot analysis of lysates from MCF7 cells, using Phospho-ESRalpha-S118 pAb (AP0146) at 1:1000 dilution. MCF7 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.