Phospho-Src-Y529 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Src-Y529 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0185
• Hersteller Artikelnummer: AP0185
• Alternativnummer: ABB-AP0185-1000UL,ABB-AP0185-20UL,ABB-AP0185-500UL,ABB-AP0185-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ASV, SRC1, THC6, c-SRC, p60-Src, Phospho-Src-Y529

Produktbeschreibung

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 60kDa
• NCBI: 6714
• UniProt: P12931
• Reinheit: Affinity purification
• Sequenz: EPQYQ
• Target-Kategorie: SRC
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|IHC-P,1:50 - 1:100|IF/ICC,1:100 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Adhesion,Cytoskeleton,Microtubules,Actins,Wnt -Catenin Signaling Pathway,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis.

Bilder

Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (AP0185) at 1:1000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.