Phospho-eIF4EBP1-T36 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-eIF4EBP1-T36 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0344
• Hersteller Artikelnummer: AP0344
• Alternativnummer: ABB-AP0344-100UL, ABB-AP0344-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: BP-1, 4EBP1, 4E-BP1, PHAS-I, Phospho-eIF4EBP1-T36

Produktbeschreibung

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Produkteigenschaften

• Molekulargewicht: 13kDa
• NCBI: 1978
• UniProt: Q13541
• Reinheit: Affinity purification
• Target-Kategorie: EIF4EBP1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Obesity,Cardiovascular,Angiogenesis.

Bilder

Western blot analysis of lysates from MDA-MB-435 cells, using Phospho-eIF4EBP1-T36 Rabbit pAb (AP0344).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.