Phospho-VEGF Receptor 2-Y1175 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-VEGF Receptor 2-Y1175 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0382
• Hersteller Artikelnummer: AP0382
• Alternativnummer: ABB-AP0382-100UL,ABB-AP0382-1000UL,ABB-AP0382-20UL,ABB-AP0382-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: FLK1, CD309, VEGFR, VEGFR2, Phospho-VEGF Receptor 2-Y1175

Produktbeschreibung

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 152kDa
• NCBI: 3791
• UniProt: P35968
• Reinheit: Affinity purification
• Sequenz: KDYIV
• Target-Kategorie: KDR
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Endocrine Metabolism,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors,Cardiovascular,Angiogenesis,Hypoxia.

Bilder

Western blot analysis of lysates from A-431 cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382) at 1:500 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.