Phospho-LIMK2-T505 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-LIMK2-T505 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0388
• Hersteller Artikelnummer: AP0388
• Alternativnummer: ABB-AP0388-100UL, ABB-AP0388-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: LIMK2, Phospho-LIMK2-T505

Produktbeschreibung

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Produkteigenschaften

• Molekulargewicht: 72kDa
• NCBI: 3985
• UniProt: P53671
• Reinheit: Affinity purification
• Target-Kategorie: LIMK2

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|IF/ICC,1:100 - 1:200
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,TGF-b-Smad Signaling Pathway.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-LIMK2-T505 Rabbit pAb (AP0388).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.