Phospho-SRC Family-Y416 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-SRC Family-Y416 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0452
• Hersteller Artikelnummer: AP0452
• Alternativnummer: ABB-AP0452-20UL,ABB-AP0452-1000UL,ABB-AP0452-100UL,ABB-AP0452-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ASV, SRC1, THC6, c-SRC, p60-Src, SRC, Phospho-SRC Family-Y416

Produktbeschreibung

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 60kDa
• NCBI: 6714
• UniProt: P12931
• Reinheit: Affinity purification
• Sequenz: NEYTA
• Target-Kategorie: Src Family
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics & Nuclear Signaling,Cancer,Signal Transduction,G protein signaling,G2/M DNA Damage Checkpoint,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Adhesion,Gap Junctions,Cytoskeleton,Microtubules,Actins,Wnt/beta-Catenin Signaling Pathway,Immunology & Inflammation,IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis,Protein phosphorylation.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0452) at 1:1000 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.