Phospho-RB-S807/811 Rabbit pAb, Unconjugated
Artikelnummer:
ABB-AP0484
- Bilder (1)
| Artikelname: | Phospho-RB-S807/811 Rabbit pAb, Unconjugated |
| Artikelnummer: | ABB-AP0484 |
| Hersteller Artikelnummer: | AP0484 |
| Alternativnummer: | ABB-AP0484-1000UL,ABB-AP0484-20UL,ABB-AP0484-100UL,ABB-AP0484-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1, Phospho-RB-S807/811 |
| The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Application Verdünnung: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,DNA Damage Repair,Protein phosphorylation,Cancer,Tumor suppressors,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G1 S Checkpoint. |
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Western blot analysis of lysates from HT-29 cells, using Phospho-RB-S807/811 Rabbit pAb (AP0484) at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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