Phospho-PKC (pan) (betaII Ser660) Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-PKC (pan) (betaII Ser660) Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0495
• Hersteller Artikelnummer: AP0495
• Alternativnummer: ABB-AP0495-1000UL,ABB-AP0495-20UL,ABB-AP0495-100UL,ABB-AP0495-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ, Phospho-PKC (pan) (betaII Ser660)

Produktbeschreibung

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 59kDa/67kDa/74- 83kDa
• NCBI: 5578
• UniProt: P17252
• Reinheit: Affinity purification
• Sequenz: YVNPQ
• Target-Kategorie: PRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Bilder

Western blot analysis of lysates from 293T cells, using Phospho-PKC (pan) (betaII Ser660) pAb (AP0495) at 1:1000 dilution or PRKCQ antibody (A5834). 293T cells were treated with PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.