Phospho-IKKalpha-S180 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-IKKalpha-S180 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0506
• Hersteller Artikelnummer: AP0506
• Alternativnummer: ABB-AP0506-100UL, ABB-AP0506-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: BPS2, IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Phospho-IKKalpha-S180

Produktbeschreibung

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Produkteigenschaften

• Molekulargewicht: 85kDa
• NCBI: 1147
• UniProt: O15111
• Reinheit: Affinity purification
• Target-Kategorie: CHUK

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling,Cardiovascular.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-IKKalpha-S180 Rabbit pAb (AP0506) at 1:1000 dilution. HeLa cells were treated with both TNF-alpha(20ng/mL) and Calyculin A (100nM) for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.