Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0538
• Hersteller Artikelnummer: AP0538
• Alternativnummer: ABB-AP0538-100UL,ABB-AP0538-20UL,ABB-AP0538-1000UL,ABB-AP0538-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: S6, eS6, Phospho-S6 Ribosomal Protein (RPS6)-S235/236

Produktbeschreibung

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 29kDa
• NCBI: 6194
• UniProt: P62753
• Reinheit: Affinity purification
• Sequenz: RLSSLR
• Target-Kategorie: RPS6
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,PI3K-Akt Signaling Pathway,MAPK-Erk Signaling Pathway.

Bilder

Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit pAb (AP0538) at 1:1000 dilution. HeLa cells NIH/3T3 cells and PC-12 cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.