Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0549
• Hersteller Artikelnummer: AP0549
• Alternativnummer: ABB-AP0549-100UL, ABB-AP0549-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: NTRK1/NTRK2, Phospho-TrkA-Y674/675 + TrkB-Y706/707

Produktbeschreibung

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Produkteigenschaften

• Molekulargewicht: 35kDa/53-60kDa/77kDa/83-93kDa
• NCBI: 4914
• UniProt: P04629
• Reinheit: Affinity purification
• Target-Kategorie: NTRK1/NTRK2

Anwendungsbeschreibung

• Application Verdünnung: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb (AP0549) at 1:500 dilution. HeLa cells were treated with Pervanadate (1 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.