Phospho-PKCdelta/theta-S643/676 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-PKCdelta/theta-S643/676 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0567
• Hersteller Artikelnummer: AP0567
• Alternativnummer: ABB-AP0567-100UL,ABB-AP0567-500UL,ABB-AP0567-20UL,ABB-AP0567-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PRKCD/PRKCQ, Phospho-PKCdelta/theta-S643/676

Produktbeschreibung

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 77kDa/80kDa/67kDa/74kDa/81kDa
• NCBI: 5580
• UniProt: Q05655
• Reinheit: Affinity purification
• Sequenz: RLSYS
• Target-Kategorie: PRKCD/PRKCQ
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation.

Bilder

Western blot analysis of lysates from RAW264.7 cells, using Phospho-PKCdelta/theta-S643/676 Rabbit pAb (AP0567) at 1:1000 dilution. RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.