Phospho-BCAR1-Y410 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-BCAR1-Y410 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0789
• Hersteller Artikelnummer: AP0789
• Alternativnummer: ABB-AP0789-100UL, ABB-AP0789-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: CAS, CAS1, CASS1, CRKAS, P130Cas, Phospho-BCAR1-Y410

Produktbeschreibung

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.

Produkteigenschaften

• Molekulargewicht: 93kDa
• NCBI: 9564
• UniProt: P56945
• Reinheit: Affinity purification
• Target-Kategorie: BCAR1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cytoskeleton,Actins.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (AP0789) at 1:5000 dilution. HeLa cells were treated with Pervanadate (1nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.