Phospho-TBC1D4-T642 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-TBC1D4-T642 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0791
• Hersteller Artikelnummer: AP0791
• Alternativnummer: ABB-AP0791-100UL, ABB-AP0791-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: AS160, NIDDM5, Phospho-TBC1D4-T642

Produktbeschreibung

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.

Produkteigenschaften

• Molekulargewicht: 147kDa
• NCBI: 9882
• UniProt: O60343
• Reinheit: Affinity purification
• Target-Kategorie: TBC1D4

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,Small G proteins,PI3K-Akt Signaling Pathway,Endocrine Metabolism,Insulin Receptor Signaling Pathway.

Bilder

Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit pAb (AP0791) at 1:2000 dilution. 293T cells were treated with IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.