Phospho-EIF4G1-S1108 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-EIF4G1-S1108 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0796
• Hersteller Artikelnummer: AP0796
• Alternativnummer: ABB-AP0796-20UL, ABB-AP0796-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, Phospho-EIF4G1-S1108

Produktbeschreibung

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Produkteigenschaften

• Molekulargewicht: 175kDa
• NCBI: 1981
• UniProt: Q04637
• Reinheit: Affinity purification
• Target-Kategorie: EIF4G1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,PI3K-Akt Signaling Pathway,Immunology Inflammation.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (AP0796) at 1:1000 dilution. NIH/3T3 cells were treated with 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.