Phospho-TSC2-T1462 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-TSC2-T1462 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0866
• Hersteller Artikelnummer: AP0866
• Alternativnummer: ABB-AP0866-20UL,ABB-AP0866-100UL,ABB-AP0866-500UL,ABB-AP0866-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: LAM, TSC4, PPP1R160, Phospho-TSC2-T1462

Produktbeschreibung

This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 201kDa
• NCBI: 7249
• UniProt: P49815
• Reinheit: Affinity purification
• Sequenz: GYTIS
• Target-Kategorie: TSC2
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Tumor suppressors,Signal Transduction,G protein signaling,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Cell cycle inhibitors,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Obesity,Immunology Inflammation.

Bilder

Western blot analysis of lysates from MCF7 cells, using Phospho-TSC2-T1462 Rabbit pAb (AP0866) at 1:1000 dilution. MCF7 cells were treated with IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.