Phospho-MST1/STK4-T387 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-MST1/STK4-T387 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0906
• Hersteller Artikelnummer: AP0906
• Alternativnummer: ABB-AP0906-20UL, ABB-AP0906-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: KRS2, MST1, YSK3, Phospho-MST1/STK4-T387

Produktbeschreibung

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and its possible that this protein induces the chromatin condensation observed in this process.

Produkteigenschaften

• Molekulargewicht: 56kDa
• NCBI: 6789
• UniProt: Q13043
• Reinheit: Affinity purification
• Target-Kategorie: STK4

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cardiovascular,Heart,Hypertrophy.

Bilder

Western blot analysis of lysates from NIH/3T3 and C6 cells, using phospho-MST1/STK4-T387 pAb (AP0906) at 1:1000 dilution. NIH/3T3 and C6 cells were treated with ATP(5 mM) at 30°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.