Phospho-mTOR-S2481 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-mTOR-S2481 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP0978
• Hersteller Artikelnummer: AP0978
• Alternativnummer: ABB-AP0978-20UL,ABB-AP0978-100UL,ABB-AP0978-500UL,ABB-AP0978-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: SKS, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, Phospho-mTOR-S2481

Produktbeschreibung

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1522]
• Molekulargewicht: 289kDa
• NCBI: 2475
• UniProt: P42345
• Reinheit: Affinity purification
• Sequenz: IHSFI
• Target-Kategorie: MTOR
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,DNA Damage Repair,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Cell Cycle,Cell cycle inhibitors,TGF-b-Smad Signaling Pathway,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Warburg Effect,Endocrine and metabolic diseases,Obesity,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis,Heart,Cardiogenesis.

Bilder

Western blot analysis of various lysates using Phospho-mTOR-S2481 (AP0978) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated with IGF-1 (50 ng/mL) at 37°C for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.