Phospho-c-Myc-S62 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-c-Myc-S62 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP0989
• Hersteller Artikelnummer: AP0989
• Alternativnummer: ABB-AP0989-100UL,ABB-AP0989-20UL,ABB-AP0989-500UL,ABB-AP0989-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: MRTL, MYCC, c-Myc, bHLHe39, Phospho-c-Myc-S62

Produktbeschreibung

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1533]
• Molekulargewicht: 51kDa
• NCBI: 4609
• UniProt: P01106
• Reinheit: Affinity purification
• Sequenz: PLSPS
• Target-Kategorie: MYC
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Cell differentiation,Cell Cycle Control-G1 S Checkpoint,Endocrine Metabolism,Warburg Effect,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Stem Cells.

Bilder

Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb (AP0989) at 1:1000 dilution. 293T cells were treated with Forskolin (10 uM) at 37°C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.