Phospho-AMPKalpha1-S496 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-AMPKalpha1-S496 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1002
• Hersteller Artikelnummer: AP1002
• Alternativnummer: ABB-AP1002-100UL,ABB-AP1002-20UL,ABB-AP1002-500UL,ABB-AP1002-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: AMPK, AMPKa1, AMPK alpha 1, Phospho-AMPKalpha1-S496

Produktbeschreibung

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1547]
• Molekulargewicht: 64kDa
• NCBI: 5562
• UniProt: Q13131
• Reinheit: Affinity purification
• Sequenz: SGSVS
• Target-Kategorie: PRKAA1
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Mouse, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Endocrine Metabolism,Mitochondrial metabolism,Lipid Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Warburg Effect,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Cardiovascular,Hypoxia,Lipids,Fatty Acids.

Bilder

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKalpha1-S496 Rabbit mAb (AP1002) at 1:1000 dilution. C2C12 cells were treated with oligomycin (0. 5 µM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.