Phospho-MEK1-S298 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-MEK1-S298 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1020
• Hersteller Artikelnummer: AP1020
• Alternativnummer: ABB-AP1020-20UL,ABB-AP1020-100UL,ABB-AP1020-500UL,ABB-AP1020-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: MEL, Phospho-MEK1-S298

Produktbeschreibung

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1565]
• Molekulargewicht: 43kDa
• NCBI: 5604
• UniProt: Q02750
• Reinheit: Affinity purification
• Sequenz: PLSSY
• Target-Kategorie: MAP2K1
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,ESC Pluripotency and Differentiation,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Warburg Effect,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Cardiovascular,Angiogenesis.

Bilder

Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (AP1020) at 1:1000 dilution. Both HeLa cells and C6 cells were treated with CIP(20uL/400ul) at 37°C for 1 hour or treated with PMA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.