Phospho-Na+/K+-ATPase-S16 Rabbit pAb

Produktübersicht

• Artikelname: Phospho-Na+/K+-ATPase-S16 Rabbit pAb
• Artikelnummer: ABB-AP1065
• Hersteller Artikelnummer: AP1065
• Alternativnummer: ABB-AP1065-100UL,ABB-AP1065-20UL,ABB-AP1065-1000UL,ABB-AP1065-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Synonym: CMT2DD, HOMGSMR2, Phospho-Na+/K+-ATPase-S16

Produktbeschreibung

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 113kDa
• NCBI: 476
• UniProt: P05023
• Reinheit: Affinity purification
• Sequenz: AVSEQ
• Target-Kategorie: ATP1A1
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,Endocrine Metabolism.

Bilder

Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065) at 1:1000 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.