This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Neuroscience, Cell Type Marker,Neuron marker,Synapse marker.
Western blot analysis of various lysates using Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit pAb (AP1080) at 1:1000 dilution. 293T cells were treated with ATP(5 mM) at 30°C for 1 hour. NIH/3T3 cells were treated with ATP(5 mM) at 30°C for 1 hour. C6 cells were treated with ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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