Phospho-MLKL-S441 Rabbit pAb

Produktübersicht

• Artikelname: Phospho-MLKL-S441 Rabbit pAb
• Artikelnummer: ABB-AP1174
• Hersteller Artikelnummer: AP1174
• Alternativnummer: ABB-AP1174-20UL, ABB-AP1174-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Synonym: 9130019I15Rik, Phospho-MLKL-S441

Produktbeschreibung

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain, however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene.

Produkteigenschaften

• Molekulargewicht: 54kDa
• NCBI: 74568
• UniProt: Q9D2Y4
• Reinheit: Affinity purification
• Target-Kategorie: Mlkl

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Rat, ResearchArea: Signal Transduction,Kinase.

Bilder

Western blot analysis of lysates from C6 cells, using Phospho-MLKL-S441 Rabbit pAb (AP1174) at 1:500 dilution. C6 cells were treated with TNF-alpha (20 ng/ml) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.