Phospho-BRD4-S1070 Rabbit pAb

Artikelnummer: ABB-AP1181
Artikelname: Phospho-BRD4-S1070 Rabbit pAb
Artikelnummer: ABB-AP1181
Hersteller Artikelnummer: AP1181
Alternativnummer: ABB-AP1181-100UL,ABB-AP1181-20UL,ABB-AP1181-1000UL,ABB-AP1181-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Alternative Synonym: CAP, MCAP, HUNK1, HUNKI, FSHRG4, Phospho-BRD4-S1070
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Klonalität: Polyclonal
Molekulargewicht: 152kDa
NCBI: 23476
UniProt: O60885
Reinheit: Affinity purification
Sequenz: IHSPQ
Target-Kategorie: BRD4
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Tumor suppressors,Signal Transduction,Kinase,Serine threonine kinases.
Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S1070 Rabbit pAb (AP1181) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.