Phospho-MCM2-S139 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-MCM2-S139 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1192
• Hersteller Artikelnummer: AP1192
• Alternativnummer: ABB-AP1192-100UL,ABB-AP1192-20UL,ABB-AP1192-1000UL,ABB-AP1192-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: BM28, CCNL1, CDCL1, cdc19, DFNA70, D3S3194, MITOTIN, Phospho-MCM2-S139

Produktbeschreibung

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 102kDa
• NCBI: 4171
• UniProt: P49736
• Reinheit: Affinity purification
• Sequenz: YDSDE
• Target-Kategorie: MCM2
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cell Biology Developmental Biology,Cell Cycle,Centrosome,Stem Cells.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (AP1192) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. HeLa cells were treated by Serum-starvation overnight at 37°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.