Phospho-ULK1-S638 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-ULK1-S638 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1196
• Hersteller Artikelnummer: AP1196
• Alternativnummer: ABB-AP1196-100UL, ABB-AP1196-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ATG1, ATG1A, UNC51, hATG1, Unc51.1, Phospho-ULK1-S638

Produktbeschreibung

Enables identical protein binding activity, protein serine/threonine kinase activity, and small GTPase binding activity. Involved in several processes, including autophagosome assembly, positive regulation by symbiont of host autophagy, and protein phosphorylation. Located in autophagosome, cytosol, and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane, extrinsic component of omegasome membrane, and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Produkteigenschaften

• Molekulargewicht: 113kDa
• NCBI: 8408
• UniProt: O75385
• Reinheit: Affinity purification
• Target-Kategorie: ULK1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Endocrine Metabolism,Mitochondrial metabolism,Neuroscience,Cardiovascular,Heart.

Bilder

Western blot analysis of lysates from 293T cells, using Phospho-ULK1-S638 Rabbit pAb (AP1196) at 1:1000 dilution. 293T cells were treated with oligomycin (0. 5 uM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.