Phospho-Acetyl CoA Carboxylase-S79 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Acetyl CoA Carboxylase-S79 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1410
• Hersteller Artikelnummer: AP1410
• Alternativnummer: ABB-AP1410-20UL,ABB-AP1410-100UL,ABB-AP1410-1000UL,ABB-AP1410-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ACC, ACAC, ACC1, ACCA, Acac1, hACC1, ACACAD, ACCalpha, ACACalpha, Phospho-Acetyl CoA Carboxylase-S79

Produktbeschreibung

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 265kDa
• NCBI: 31
• UniProt: Q13085
• Reinheit: Affinity purification
• Target-Kategorie: ACACA/ACACB
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Mouse, ResearchArea: ACC, ACAC, ACC1, ACCA, Acac1, hACC1, ACACAD, ACCalpha, ACACalpha.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Acetyl CoA Carboxylase-S79 Rabbit PAb (AP1410) at 1:1000 dilution. NIH/3T3 cells were treated with oligomycin (0. 5 uM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.