Phospho-AKT1S1/PRAS40-T246 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-AKT1S1/PRAS40-T246 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1493
• Hersteller Artikelnummer: AP1493
• Alternativnummer: ABB-AP1493-500UL,ABB-AP1493-1000UL,ABB-AP1493-20UL,ABB-AP1493-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: Lobe, PRAS40

Produktbeschreibung

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).

Produkteigenschaften

• Molekulargewicht: 27 kDa
• NCBI: 84335
• UniProt: Q96B36
• Reinheit: Affinity purification
• Sequenz: LNT(p)SD
• Target-Kategorie: AKT1S1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:2000 - 1:9000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,Neuroscience.

Bilder

Western blot analysis of lysates from HeLa cells using Phospho-AKT1S1/PRAS40-T246 Rabbit mAb (AP1493) at 1:8000 dilutionincubated overnight at 4°C. HeLa cells were treated with Insulin (50 nM) at 37°C for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60 s.