Phospho-NFKB1-S337 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-NFKB1-S337 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1528
• Hersteller Artikelnummer: AP1528
• Alternativnummer: ABB-AP1528-20UL,ABB-AP1528-1000UL,ABB-AP1528-500UL,ABB-AP1528-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta

Produktbeschreibung

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Produkteigenschaften

• Molekulargewicht: 85kDa/105kDa
• NCBI: 4790
• UniProt: P19838
• Reinheit: Affinity purification
• Sequenz: RKSDL
• Target-Kategorie: NFKB1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:3000 - 1:18000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway.

Bilder

Western blot analysis of lysates from HeLa cells using Phospho-NFKB1-S337 Rabbit mAb (AP1528) at 1:3000 dilutionincubated overnight at 4°C. HeLa cells were treated with TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.