Phospho-YWHAZ -Ser58+YWHAH -Ser59+YWHAG-Ser59+YWHAB -Ser60 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-YWHAZ -Ser58+YWHAH -Ser59+YWHAG-Ser59+YWHAB -Ser60 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1561
• Hersteller Artikelnummer: AP1561
• Alternativnummer: ABB-AP1561-100UL,ABB-AP1561-1000UL,ABB-AP1561-500UL,ABB-AP1561-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: HEL4, YWHAD, KCIP-1, HEL-S-3, POPCHAS, HEL-S-93, 14-3-3-zeta,YWHA1,14-3-3GAMMA, DEE56, EIEE56,PPP1R170,GW128,HEL-S-1,HS1,KCIP-1,YWHAA,

Produktbeschreibung

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5 UTR but that encode the same protein have been identified for this gene.

Produkteigenschaften

• Molekulargewicht: 28kDa
• NCBI: 7534
• UniProt: P63104
• Reinheit: Affinity purification
• Sequenz: RSSWR
• Target-Kategorie: YWHAZ/YWHAH/YWHAG/YWHAB

Anwendungsbeschreibung

• Application Verdünnung: WB,1:6500 - 1:13000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Signal Transduction,PI3K-Akt Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint,Cytoskeleton,Actins,Immunology Inflammation,Neuroscience, Cell Type Marker,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Neurodegenerative Diseases Markers.

Bilder

Western blot analysis of various lysates using Phospho-YWHAZ -Ser58+YWHAH -Ser59+YWHAG-Ser59+YWHAB -Ser60 Rabbit mAb (AP1561) at 1:13000 dilutionincubated overnight at 4°C. HeLa cells and NIH/3T3 cells were treated with IBMX (50µM) and Forskolin (10 µM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.