Phospho-Raptor-Ser792 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Raptor-Ser792 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1578
• Hersteller Artikelnummer: AP1578
• Alternativnummer: ABB-AP1578-100UL,ABB-AP1578-500UL,ABB-AP1578-1000UL,ABB-AP1578-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: A synthetic phosphorylated peptide around S792 of human RPTOR (NP_065812.1).
• Konjugation: Unconjugated
• Alternative Synonym: KOG1, Mip1

Produktbeschreibung

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

Produkteigenschaften

• Molekulargewicht: 149 kDa
• NCBI: 57521
• UniProt: Q8N122
• Reinheit: Affinity purification
• Sequenz: ASSYS
• Target-Kategorie: RPTOR

Anwendungsbeschreibung

• Application Verdünnung: WB,1:5000 - 1:20000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensur
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Signal Transduction,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Cell Cycle,Cell cycle inhibitors,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway.

Bilder

Western blot analysis of lysates from 293F cells using Phospho-Raptor-Ser792 Rabbit mAb (AP1578) at 1:5000 dilutionincubated overnight at 4°C. 293F cells were treated by Oligomycin (0.5 µM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.