Phospho-Crk p38-Y221 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Crk p38-Y221 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1585
• Hersteller Artikelnummer: AP1585
• Alternativnummer: ABB-AP1585-20UL,ABB-AP1585-100UL,ABB-AP1585-1000UL,ABB-AP1585-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: p38, CRKII

Produktbeschreibung

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Produkteigenschaften

• Molekulargewicht: 34 kDa
• NCBI: 1398
• UniProt: P46108
• Reinheit: Affinity purification
• Sequenz: GPYAQ
• Target-Kategorie: CRK

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation.

Bilder

Western blot analysis of lysates from K-562 cells using Phospho-Crk p38-Y221 Rabbit mAb (AP1585) at 1:5000 dilutionincubated at room temperature for 1.5 hours. K-562 cells were treated with lambda-PP mixed solution (1 µL) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.